Curcumin inhibits hyperoxia-induced pulmonary fibrosis in neonatal rats / 医学研究生学报

ABSTRACT

Objective:To observe the effect of curcumin(Cu) on collagen deposition and the expression of transforming growth factor-?1(TGF-?1) in neonatal rats with hyperoxia-induced pulmonary fibrosis.Methods: A total of 160 term neonatal rats within 12 hours after birth were equally randomized into Group 1(air-exposed),2(air-exposed + Cu-treated),3(hyperoxia-exposed) and 4(hyperoxia-exposed + Cu-treated).The neonatal rats in Groups 3 and 4 were kept in the chambers containing 90% concentration oxygen and intraperitoneally injected with curcumin(200mg/) in the oleum lini solution,while those in Groups 1 and 2 were exposed to room air and intraperitoneally given the same dose of oleum lini only.Five rats were randomly taken from each group and sacrificed at 3,7,14 and 21 days,respectively,after the treatment,their lungs incised for HE and Masson staining and the protein expressions of TGF-?1 detected by immunohistochemistry.Results: A great deal of collagen deposition was observed in the bronchus submucosa,the vessel wall and its surroundings,the alveollar wall and the lung interstitium in Group 3,less obvious in Group 4,and the least obvious in Groups 1 and 2.The expression of TGF-?1 was significantly higher in Group 3 than in Group 4(P