Effects of inflammatory mediator blocker on neurological deficits,apoptosis and expression of caspase-3 following cerebral ischemia-reperfusion in rats / 临床神经病学杂志

ABSTRACT

Objective To explore the effects of inflammatory mediator blocker AG490 on neurological deficits,apoptosis and expression of caspase-3 following cerebral ischemia-reperfusion(I/R) in rats.Methods The male SD rats were randomly divided into the groups sham-operation,I/R,saline and AG490;and the focal cerebral I/R models were made by middle cerebral artery thread embolism method.AG490(1 mg/kg) was intraperitoneal injection in AG490 group immediate and 12 h after ischemia-reperfusion respectively.The neurological deficits score was evaluated in 24 h after I/R in each group.The number of apoptosis in cerebral tissue was examined by d-utp nick end labeling staining(TUNEL).The expression of P-JAK2,P-STAT3,caspase-3 were detected by Western Blot.Results Compared with the groups I/R and saline,the neurological deficits score in AG490 group was significantly decreased(all P