The effect of STAT3 decoy ODNs on the growth and apoptosis of Hep G2 cells / 临床检验杂志

ABSTRACT

Objective To investigate the effect of STAT3 decoy ODNs on the growth and apoptosis of hepatoma cells Hep G2 and the related mechanism.Methods STAT3 decoy ODNs,synthesized in vitro,was transferred into Hep G2 cells by cationic lipid.The cell growth curve was used to reflect the growth and proliferation capacity of Hep G2,and annexin-V/PI staining method was used to calculate apoptic cells by FCM.Real time-PCR was used to quantitatively detect the expression of bcl-xL,cyclin D1,and c-myc mRNA in Hep G2.Results FCM assay and fluorescence microscopy demonstrated that STAT3 decoy ODNs was successfully transferred into Hep G2 cells(90.2%).Decoy ODNs inhibted the growth of Hep G2 cells.After treated with decoy ODNs 48 h,the percentage of early apoptotic cells was(22.86 ? 2.63)% and that of late apoptotic cells was(23.00 ? 2.76)%.Real time-PCR indicated that Hep G2 cells transfected with STAT3 decoy ODNs had decreased the mRNA expression of bcl-xL,cyclin D1 and c-myc.Conclusions STAT3 decoy ODNs inhibited the growth of Hep G2 cells and induce the apoptosis.The mechanism may be associated with decreased expression of bcl-xL,cyclin D1 and c-myc mRNA.