IFN-? Release Assays for Rapid Diagnosis of Active Tuberculosis:Clinical Application / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To evaluate the clinical application of T-cell based IFN-? release assays(IGRA) for the rapid diagnosis of active tuberculosis.METHODS IFN-? and HIV antibody were detected by using ELISA.Antibody to Mycobacterium tuberculosis was detected by colloidal gold.At the same time,the M.tuberculosis DNA loads were examined by FQ-PCR.Statistical analysis were performed to analyze the correlation of IFN-? with M.tuberculosis antibody and DNA,respectively.RESULTS The sensitivity of TB-IGRA was 90.24%,specificity was 93.34%;the positive rate of TB-IGRA in 82 tuberculosis patients was higher than from sputum smear(64.63%),TB-PCR(76.83%) and tuberculosis antibody(40.24%).CONCLUSIONS As a replacement of TB-PCR,IFN-? can be used as a valued index to evaluate tuberculosis infectin.