Inhibitory effect of ethanol extract from Java Brucea Fruit on cell migration mediated by platelet-derived growth factor receptor ? / 吉林大学学报(医学版)

ABSTRACT

Objective To study the effects of ethanol extract from Java Brucea Fruit on platelet-derived growth factor receptor ?(PDGFR?) mediated cell migration and to obtain the valuable messages on the characteristics of its active ingredient.Methods PAE cells was transfected with the vector expressing human PDGFR? with Transfectom 2000;After screening by G418 resisitance,RT-PCR was used to monitor the expression of PDGFR? in the cells;Wound healing of the cells was used to examine the lowest consistency of PDGFBB and inhibitory effect of ethanol extract of Java Brucea Fruit on cell migration after restoring 24 h.Results Human PDGFR? was stably expressed in PAE cells transfected with the expressing vector.The lowest consistency of exogenous PDGFBB which promoted PDGFR? mediated cell migration was 10 ?g?L-1.70% ethanol extract of Java Brucea Fruit which strongly inhibited PDGFR? mediated cell migration was dose-dependent(P10 ?g?L-1) mainly caused the death of the cell.Conclusion Ethanol extract of Java Brucea Fruit has a strongly inhibitory effect on the PDGFR? mediated cell migration which could play a major role in its effects against metastasis of malignant tumor,the active ingredients of it could be more dissolvable in the 70% ethanol.