Change of interleukin-23 level in mouse asthma model and its action mechanism / 吉林大学学报(医学版)

ABSTRACT

Objective To measure the change of serum interleukin-23(IL-23) level before and after treatment with glucocortcoid and analyze the correlations between levels of IL-23 and IL-4,IFN-?,IL-17,and explore the role of IL-23 in bronchial asthma.Methods Thirty femal BALB/c mice were randomly divided into three group(n=10) group A(asthma model),group B(corticosteroid treatment) and group C(normal control).All mice were killed 24 h after final OVA challenge.The blood samples were obtained for measurements of serum IgE,IL4,IFN-?,IL-17and IL-23 levels by enzyme-linked immunoabsorbent assay(ELISA).Results The mouse asthma model appeared ethological changes specific to asthma.The level of OVA specific IgE in serum increased.The level of serum IL-4 increased and the level of IFN-? decreased.Correlation analysis showed negative relationship between IL-4 and IFN-?(r=-0.859,P