Determination of major nucleosides in Hyriopsis cumingii L. by HPLC / 中国海洋药物
Chinese Journal of Marine Drugs
; (6)1994.
Article
in Zh
| WPRIM
| ID: wpr-595509
Responsible library:
WPRO
ABSTRACT
Objective A HPLC method was established to measure the contents of major nucleosides in Hyriopsis cumingii.Method Separation was performed on a Lichrosper-C_(18) column, pure water was used as mobile phase,the flow rate was 1.0mL min~(-1),the column temperature at 30℃and the detective walvelength at 254 nm.Results The contents of uracil,hypoxanthine, xanthine,uridine and thymine were 153.2~173.3?g?g~(-1),106.6~360.8?g?g~(-1),117.6~172.8?g?g~(-1),70.3~153.1?g?g~(-1) and 343.9~511.4?g?g~(-1),respectively. Conclusion This method showed good repeatability and flexibility.It could be used as a conventional method for determination of major nucleosides in H.cumingii.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Marine Drugs
Year:
1994
Type:
Article