Effects of Laurencia terpenoids on the levels of IL-6 and TNF-?in H_(22) mice / 中国海洋药物

ABSTRACT

Objective To study the effects of Laurencia terpenoids(LET)on the levels of IL-6 and TNF-?in H_(22) mice.Methods 1.The content of total terpenoids in LET was detected by RP-HPLC.2.The mice were randomly divided into five groupsthe model group,the LET groups(25,50,100mg?kg~(-1)?d~(-1)) and the Cyclophosphamide(CY) group,and transplant tumor model of hepatoma 22(H_(22)) was established.The next day four groups of animal(except the model group) were treated with different dosage of LET and CY(ig) and executed after 15 days.The tumors were weighed and the tumor inhibition rates were calculated.Furthermore the levels of IL-6 and TNF-?in serum were determined by RIA.Results In vivo, the increases of H_(22) tumor weight in LET groups were slower than that in model group.The tumor inhibition rates were 27.5%,34.9%and 41.4%correspondently.Mid and high-dose groups of LET could increase the levels of IL-6 and TNF-?.Conclusion LET could inhibit the increase of H_(22) tumor weight.The mechanism may be related to enhancing the level of cytokine including IL-6 and TNF-?.