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Expressions of 11?-hydroxysteroid dehydrogenase Types 1 and 2 in colorectal cancer cells and effects of glucocorticoid and glycyrrhetinic acid on this cell line / 医学研究生学报
Journal of Medical Postgraduates ; (12)2003.
Article in Chinese | WPRIM | ID: wpr-595577
ABSTRACT

Objective:

The 11?-hydroxysteroid dehydrogenase(11?-HSD) plays an important role in tumor biological behavior,and the 11?-HSD inhibitor Glycyrrhetinic acid(GA) may have an anticancer effect,although its mechanism remains unkown.This study aimed to observe the expressions of 11?-HSD 1 and 2 in colorectal cancer and the effects of glucocorticoid and GA on colorectal cancer cells.

Methods:

The mRNA expressions of 11?-HSD1 and 2 in the human colorectal cancer cell line HCT-8 were detected by reverse transcription polymerase chainreaction(RT-PCR),and the protein expressions of 11?-HSD1 and 2 were detected by Western blot.The inhibition of the growth of the HCT-8 cells was determined by MTT assay after treated with glucocorticoid,cosubstrate,GA only or their combination.

Results:

The expression of 11?-HSD2 mRNA and proteins but not that of 11?-HSD1 mRNA and proteins was expressed in the HCT-8 cells.The rate of cell growth inhibition was(40.87 ? 1.47)% after a 12-hour treatment with cortisol,and it was(5.79 ? 0.20)% in the cortisol + NAD group,with statistically significant difference(P

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Medical Postgraduates Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Medical Postgraduates Year: 2003 Type: Article