Effects of AP-2? on invasive growth and ER-? expression of colon cancer cell line / 中国肿瘤生物治疗杂志

ABSTRACT

Objective: To study the effects of transcription factor activator protein-2?(AP-2?)on invasive growth and estrogen receptor-?(ER-?) expression in human colon cancer SW620 cells,and to probe into the involved molecular mechanism.Methods: Plasmid pcDNA3.1(+)-AP-2? and pcDNA3.1(+) were transfected into SW620 cells by liposome-mediated transfection.The adhesion,invasion and migration abilities of SW620 cells were measured by metrical gel adhesion assay and modified Boyden chamber(Transwell assay).The gene and protein expression levels of AP-2? and ER-? in SW620 cells were examined by Real-time PCR,Western blotting and immunofluorescence cytochemistry.The interaction between AP-2? DNA and ER-? in SW620 cells was measured by electrophoretic mobility shift assay(EMSA) after AP-2? gene transfection.Results: Overexpression of AP-2? markedly reduced the adhesion,invasion and migration abilities of SW620 cells(all P