In vitro Activity of Ertapenem and Other Antibiotics Against Extended-spectrum Beta-lactamase Producing Escherichia coli and Klebsiella pneumoniae / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To analyze in vitro activity of ertapenem and other antibiotics against clinical bacterial isolates of ESBLs-producing Escherichia coli and Klebsiella pneumoniae.METHODS E.coli and K.pneumoniae were identified by WalkAway40 with 21CN.ESBLs-producing isolates confirmed accordance with the CLSI guidelines.E-test strips were used for determining the minimum inhibitory concentration(MIC) of ertapenem,while disk diffusion test was performed on each isolate using 10 ?g ertapenem disc.RESULTS ESBLs-producing strains were isolated mainly from sputum,urine,secretion and blood,most of them were from nephrology,neurosurgery,Respiratory department and ICU.High resistance rates were shown for both E.coli and K.pneumoniae against cefepime,ciprofloxacin(63.42%),SMZco(61.78%),tobramycin(45.22%)and gentamicin(61.14%).Most K.pneumoniae and E.coli isolates were susceptible to amikacin(78.70%) and piperacillin/tazobactam(81.30%).Tmipenem showed 100.00% of activity the isolates.Ertapenem showed activity against 100.00% ESBLs-producing E.coli,one ESBLs-producing K.pneumoniae isolate showed ertapenem resistance and another one intermediate,the ratio of susceptibility was 99.40%,ertapenem had MIC at which 50.00% and 90.00% of the isolates were inhibited of 0.032 ?g /ml and 1.0 ?g /ml for ESBLs-producing organisms respectively.CONCLUSIONS Ertapenem is less active to ESBLs-producing organisms then imipenem.Ertapenem resistance appears to be the most sensitive indicator for detection of KPCs.