Construction of eukaryotic expression vector containing telomere/telomerase binding factor PinX1 and its expression in Hek293 cells / 临床检验杂志

ABSTRACT

Objective To study the cellular distribution of PinX1 protein in transfected Hek293 cells.Methods The PinX1 mRNA was amplified from HepG2 cells by RT-PCR and inserted into pcDNA3-vsv vector,and the vector was transfected into Hek293 cells. The expressed protein was detected by immunocytochemical method.Results PinX1 mRNA from HepG2 was obtained and the recombinant vector pcDNA3-PinX1-vsv was successfully constructed.Immunocytochemical method verified that PinX1 was expressed in nuclei after transfected.Conclusion We successfully got PinX1 mRNA,and it could be expressed in nuclei of Hek293 cells.This sets up a solid foundation for PinX1's function study of mediating telomere and telomerase.