Culturing and Identification of Penicillium marneffei / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To improve the diagnosis level of Penicillium marneffei culture identification in the grass-roots laboratories.METHODS The patient′s blood or bone marrow were innoculated to BacT/Alert 3D blood culture system.when positive sample was found,the culture fluid and node tissues were smeared to take,Gram stain.The pathogens suchas small long branch,septate hyphae,sausage-shaped cell,were transferred to Colombia blood plate,sand Paul agar and CHROMagar Candida chromogenic culture medium to observe the 25 ℃ and 35 ℃,to observe the colony morphology and cell characteristics.The relevant biochemical reaction tests,were cutler taken.RESULTS The foundation of identifying P.marneffei cultureit is a dimorphic fungus,cultured for fungal phase at 25℃,and for yeast phase at 35℃.Both could produce wine red pigment.Morphology under the microscopeculture had typical broom-shaped sticks at 25℃,and round,oval,blunt and slightly curved at both ends,and sausage-shaped thallus that had horizontal septum.Biochemical results:both could assimilate and ferment glucose to produce acid,urea luciferase positive.Both could be inhibited by Actidione,and couldn′t assimilate fermenting lactose.CONCLUSIONS The appropriate medium for the P.marneffei is Sabourand′s medium.The appropriate stains of the cell morphology and structure are lactic acid cotton blue staining and Gram staining which are suitable for the grass-root level laboratories.