Chondrocytic differentiation of bone marrow mesenchymal stem cells induced by Ad-hTGF-?1 / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53)2007.
Article
in Chinese
| WPRIM
| ID: wpr-596538
ABSTRACT
BACKGROUND:
Tissue engineering development brings a hope for articular cartilage defect repair.Current studies have demonstrated that bone marrow mesenchymal stem cells (MSCs) are the best cell source to repair full-thickness cartilage defects.OBJECTIVE:
To induce MSCs to differentiate into chondrocytes with Ad-hTGF-?1 in pellet culture system in vitro and identify the differentiated cells.DESIGN,TIME ANDSETTING:
Repetitive cellular measurements were performed at the Central Laboratory of Xinqiao Hospital,Third Military Medical University of Chinese PLA from June 2007 to January 2008.MATERIALSJapanese rabbits,2 months old,were provided by the Laboratory Animal Center of Third Military Medical University of Chinese PLA.METHODS:
The rabbit MSCs were isolated,cultured and expanded in vitro.After transfected with Ad-hTGF-?1,the cells were cultured in pellet culture system.Chondrogenic differentiation was evaluated by histological,immunohistochemical and RT-PCR techniques.MAIN OUTCOMEMEASURES:
Cell morphological changes were observed by histological staining;proteoglycan and type II collagen expression was detected by immunohistochemical and RT-PCR techniques.RESULTS:
The induced cells exhibited a chondrocyte-like morphology by histological staining.Immunohistochemical and RT-PCR results showed that proteoglycan and type II collagen were expressed in the induced cells.CONCLUSION:
Bone marrow MSCs cultured in pellet culture system can differentiate into chondrocytes under the induction of Ad-hTGF-?1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2007
Type:
Article
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