Quinolone-resistance Genes in Multi-Drug-Resistant Klebsiella pneumoniae / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To investigate the quinolone-resistance mechanisms of multi-drug-resistant Klebsiella pneumoniae(MDRKP).METHODS Seven kinds of chromosome and plasmid mediated quinolone-resistance genes were analyzed by PCR and verified by DNA sequencing in 25 strains of MDRKP.RESULTS In 25 strains of MDRKP,the positive rate of genes of gyrA,aac(6′)-Ⅰb-Cr,qnrA1,qnrB4-like,qnrS1,mdfA,and qepA were 76.0%,36.0%,8.0%,8.0%,12.0%,100.0%,and 0,respectively.CONCLUSIONS The mutation of gyrA gene is the main cause of the resistance of quinolone in the 25 strains of MDRKP.