A preliminary experimental study of proximal regeneration process of sciatic nerve after injury / 中华显微外科杂志

ABSTRACT

Objective GAP-43 protein is a marker of peripheral nerve regeneration,To report the regeneration of rat proximal sciatic nerve were observed labeled by GAP-43 in two types of surgical repair model. Methods Right side of sciatic nerves of 64 SPF-class healthy adult male SD rats were cut at 5cm above the branch,32 rats were subjected to small gap(2mm)bridging repair with chitin conduit;others were subjected to traditionary epineurium suture.The general conditions of local tissue,sciatic nerve growth state and regenerative patterns of peripheral nerve fiber were observed at 1 d,3 d,7 d and 14 d respectively.Results The small gap bridging group had less local adhesion,immunofluorescencc staining showed that new axons Was in conic-sharp and homogeneously tidy.Local adhesion of traditionary epineurium suture group was more serious,distal new regenerated axons were irregular compared to the small gap bridging group.Immunofluorescence image analysis results showed that early after the repair of rat sciatic nerve injury.the number of new axons of traditionary epineurium suture group was more compared with small gap bridging group(P<0.01);around 14 days after operation,the number of small gap bridging group was significantly higher than the traditionary epineurium suture group(P<0.05). Conclusion The small gap bridging group has less focal adhesion, better shape of nerve fibers and more regenerating axons than the traditionary epineurium suture group.The regenerative law of peripheral nerve fibers may further give us an explanation why regeneration results of small gap bridging group is better than traditionary epineurium suture group.