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Effects of erythropoietin on Schwann cells in vitro / 中华显微外科杂志
Chinese Journal of Microsurgery ; (6): 381-383,illust 4, 2009.
Article in Chinese | WPRIM | ID: wpr-597047
ABSTRACT
@#Objective To observe the effects of EPO on GDNF secretion and cell cycle of Schwann cells(SCs) in vitro,and explore how EPO improve peripheral nerve regeneration. Methods Devided the purified Schwann cells of primary culture into three groupsgroup A with DMEM culture solution contained 10%fetal bovine serum and without EPO,group B with DMEM culture solution as above and 10 U/ml EPO,group C with DMEM culture solution as above and 50 U/ml EPO.The GDNF level of each group was detected in the culture by ELISA,and carried out the flow cytometry of Schwann cells on each group.Results GDNF in culture solution of group B,group C was more than that of group A,and the S-stage rate(S%)and (S+G_2M)%of Schwann cells in group B and group C more than that of group A. Conclusion EPO can increase the GDNF secretion and enhance proliferation activity of Schwann cells,which can explain how EPO improve peripheral nerve regeneration.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Microsurgery Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Microsurgery Year: 2009 Type: Article