Schwann cells induced neuronal differentiation of adipose-derived stem cells (ADSCs) / 中华显微外科杂志
Chinese Journal of Microsurgery
;
(6): 119-122,illust 5, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-597105
ABSTRACT
@#Objective To investigate the function of SD rats Schwann cells on the growth and differentiation of adipose-derived stem cells(ADSCs) when they are co-cultured in Transwell culture system. Methods The ADSCs harvested from SD rats were divided into 3 groups. Group A ADSCs and Schwann cells were cultured in Transwell indirect co-culture system. Group B ADSCs were induced by β-BME and BHA. Group C a contrast group. The morphology of ADSCs was observed and immunohistology was pedormed. Results ADSCs of group A and group B were partly differentiated to the cells which had long neurite,and had a positive result in the NF staining, but negative in the GFAP staining. Group A shows no great difference in neurite length compared with group B(P < 0.05), and no great difference in cell quantity compared with group C (P < 0.05). Conclusion Schwann cells of SD rat have a growth supportive and neuronal differentiation inducing effect on ADSCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Microsurgery
Year:
2009
Type:
Article
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