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Establishing of the HSV-2 infected cell culture system / 中国基层医药
Chinese Journal of Primary Medicine and Pharmacy ; (12): 11-13,插6, 2010.
Article in Chinese | WPRIM | ID: wpr-597254
ABSTRACT
Objective To establish a HSV-2 infected cell culture system,so as to confirm the suppressing effect of medicine on HSV-2 including effective concentration and safety.Methods A monkey kidney cell line,Vero ,and a human tracheal epithelial cell line,Hep-2 were used in HSV infected cell culture system.HSV-2 was propagated in Vero cells and Hep-2 cells was served as targets of HSV-2 infection.Then cytopathic effect(CPE) was performed.Results HSV-2 was propagated abundance in Vero cells after4 daysThe CPE occurred in Hep-2 cells after 12 hours infected by HSV-2.Conclusion The HSV-2 infected cell culture system we established was stable for utilization.As well as to confirm the suppressing effect of medicine on HSV-2.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Primary Medicine and Pharmacy Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Primary Medicine and Pharmacy Year: 2010 Type: Article