Experimental study on the chondroyte-like differentiation of marrow mesenchymal stem cell

ABSTRACT

BACKGROUND:

Tissue engineering method has been employed to recover cartilage defect and overcome many traditional shortages.

In vitro marrow mesenchymal stem cells (MSCs) of adult rat was induced to differentiate into chondrocyte phenotype so as to probe into the feasibility of MSCs to be cartilage seed cell in tissue engineering.

Completely randomized design and controlled experimental study.

Department of Histology and Embryology, and Department of Neurobiology, Harbin Medical University MATERIALS Six Wistar rats of either gender, cleanness grade, were provided by Experimental Animal Center, Affiliated Second Hospital, Harbin Medical University. Permission number of experimental animal production was SCXK(black) 20020002.

The MSCs of the second generation adult rat was taken and divided into test group and control group. The test group was induced with serum free and control group was induced with 10% fetal calf serum. Collagen type Ⅱ immunohistochemical staining, toluidine blue staining was performed to detect differentiation.MAIN OUTCOME

Identification of chondrocyte, comparison of the positive rate of Collagen type Ⅱ immunohistochemical staining at different induction time point.

Induced MSCs had identical characteristic to the chondrocyte.

In vitro culture can induce MSCs to differentiate directly to chondrocyte-like cell. The results suggest that it is feasible to use MSCs as seed cells in the cartilage tissue engineering.