Expressions of SUMO4, NF-κB and IκB in kidney of rats with type 2 diabetes mellitus and its significance / 心血管康复医学杂志
Chinese Journal of cardiovascular Rehabilitation Medicine
;
(6): 1-5, 2012.
Article
in English
| WPRIM
| ID: wpr-597769
ABSTRACT
Objective:
To study expressions of small ubiquitin-related modifier protein(SUMO)4 (SUMO4), nuclear factor (NF)- κB and inhibitory factor of NF-κB (IκB) in kidneys of rats with type 2 diabetes mellitus (T2DM).Methods:
A total of ten 40-week-old male Goto-Kakizaki (GK) rats (with spontaneous diabetes mellitus)of specific-pathogen free (SPF) grade, and ten 40-week-old male Wistar rats of SPF grade were selected. The lesion of renal tissue was observed by hematoxylin eosin (HE) staining. Expressions of SUMO4, NF-κB and IκB in renal tissue were observed by immunohistochemistry methods.Results:
In the GK rats, glomerular capillary ball hypertrophy, basilar membrane slightly thickening; glomerular mesangial cells hyperplasia, hypertrophy and renal tubular epithelial cells hypertrophy were observed. Compared with normal Wistar rats, expression levels of NF-κB [(0.232±0.034) vs. (0.634±0.058)], IκB [(0.242±0.027) vs. (0.712±0.078)] and SUMO4 [(0.160±0.031) vs. (0.545±0.045)] significantly increased in renal tissue of GK rats (P<0.01 all).Conclusion:
Compared with Wistar rats, expressions of NF-κB, IκB and SUMO4 significantly increase in renal tissue of GK rats, suggesting that SUMO inhibiting transcriptional activity of NF-κB may exist in kidneys of T2DM rats. Therefore, sumoylation may be a new therapeutic target for inhibit renal microvascular lesion of diabetic disease.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Chinese Journal of cardiovascular Rehabilitation Medicine
Year:
2012
Type:
Article
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