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The effect of let-7b on melanoma cell proliferation and aerobic glycolysis / 中国医师杂志
Journal of Chinese Physician ; (12): 577-579,584, 2012.
Article in Chinese | WPRIM | ID: wpr-598036
ABSTRACT
Objective To study the influence of let-7b on cell proliferation and aerobic glycolysis of human melanoma cell A375.Methods Transfect A375 cell line with hsa-let-7b oligonucleotide or antisense.Glucose and lactate in medium were determined by spectrophotometry at 24 h and 48 h time point after transfection.The cell proliferation was determined by methylthiazol tetrazolium (MTT) assay.Results Over expression of let-7b in melanoma cell reduced cell proliferation notably,compared to the other groups by MTT(P <0.05).However,the glucose consumption and lactate production differences were not observed during 24 h or 48 h ( P > 0.05 ),the blank control group transformed about 57% and 43% glucose to lactate during 24 h and 48 h.Conclusions Melanoma cell line A375 has notably aerobic glycolysis hallmark,let-7b could inhibit proliferation of melanoma cell line A375,but it may has no influence on glucose metabolism.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Chinese Physician Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Chinese Physician Year: 2012 Type: Article