Chemokine Receptor 5 of Human Brain Microvascular Endothelial Cells Takes Part in Amyloid beta Induced T Lymphocytes Transendothelial Migration / 中国医科大学学报
Journal of China Medical University
;
(12): 410-412,431, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-598240
ABSTRACT
Objective To investigate the function of chemokine receptor 5(CCR5)in human brain microvascular endothelial cells(HBMEC)during T lymphocytes transendothelial migration induced by amyloid beta(Aβ).Methods The in vitro model of blood-brain barrier was established with cultured HBMEC monolayer.The ability of T lymphocytes transendothelial migration induced by Aβ was analyzed by Transwell and Millicell-ERS endothelia volt-ohmmeter.Results Aβ could promote T lymphocytes to migrate through the HBMEC monolayer.Over-expression of CCR5 in HBMEC promoted T lymphocytes to migrate through HBMEC monolayer.Expression of CCR5 mutant in HBMEC inhibited the transendothelial migration of T lymphocytes.Conclusion CCR5 of HBMEC is involved in T lymphocytes transendothelial migration induced by Aβ.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of China Medical University
Year:
2010
Type:
Article
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