Promoting Effect of Schwann＇s Cells on the Differentiation of Rat Bone Marrow-derived Mesenchymal Stem Cells into Neuron-like Cells / 中国医科大学学报

ABSTRACT

Objective To approach a new method induce the differentiation of mesenchymal stems cells（MSC）into neuron-like cells.Methods We co-cultured Schwann cells with MSC to induce the differentiation of rat MSC into neuron-like cells.Immunohistochemistry was employed to observe the expression of NSE and Brdu,and Western blot to examine the expression of NeuN by in transdifferenciated MSC（Neuron-like cells）.Additionally,we analyzed the differentiating rate of MSC.Results The co-culture of MSC with Schwann cells could induce the expression of NSE and NeuN.They have been differentiated into neuron-like cells.The differentiating rate of MSC was（80.51±5.65）%.Conclusion The co-culture with Schwann′s cells could differentiate rat bone marrow derived MSC into Neuron-like cells.