The effect of resveratrol on acute gouty arthritis / 中华风湿病学杂志

ABSTRACT

Objective To study the effects of resveratrol on acute gouty arthritis.Methods According to random number table,thirty-six Wista rats were randomly divided into six groupsthe control group,the model group,the colchicine treatment group,the high dose resveratrol group,the medium dose resveratrol group,the low dose resveratrol group.They were administrated with normal saline,colchicine suspension,high dose resveratrol,medium dose resveratrol,low dose resveratrol for 7 days once daily.Acute gouty arthritis rats models were established on the fourth day by injecting monosodium urate solution (concentration of 25 mg/ml,0.05 ml) into the ankle joint,while 0.05 ml normal saline was injected into the joint of the control rats.Seventy-two hours after the model was established,the synovial fluid were collected from ankle joints for the measuring of the level of IL-1β,CXCL10.NF-κB p65 protein levels were assayed with Western blotting.The joint synovia were fixed for histopathological examination with 10％ fromaldehyde.Variance analysis were used.Results The values of IL-1β in the low,medium and high doses of resveratrol were (9.63±0.71),(7.67±0.48) and (6.66±0.29) pg/ml respectively,being significantly lower than that in the model group (11.85±0.45) pg/ml (P＜0.05).The values of CXCL10 in the medium and high doses of resveratrol were (86.8±2.9) and (89.6±5.5) pg/ml respectively,being significantly lower than that in the model group (110.1±4.0) pg/ml (P＜0.05).The Western blotting results showed that the expression of NF-κB p65 protein in the high dose resveratrol group decreased evidently than that in the model group.The histopathological examination showed that resveratrol might reduce joint edema,decrease inflammatory cells infiltration in acute gouty arthritis.Conclusion The levels of IL-1β,CXCL10 and the expression of NF-κB p65 protein are elevated in acute gouty arthritis.The resveratrol can effectively control acute gouty arthritis attacks,and its efficacy is dose dependent.