Expression of cholecystokinin receptor B in a variety of human tumor tissues and cells / 重庆医学

ABSTRACT

Objective To investigate the expression of cholecystokinin receptor B(CCK-BR)in a variety of human tumor tissues and cells.Methods RT-PCR and Western Blot were used to detect the expression of CCK-BR mRNA and protein in human gastric cancer AGS and SGC-7901cells,human hepatoma cancer HepG-2 cells,human neuroblastoma SH-SY5Y cells and human lung canc-er A549 and NCI-H446 cells,human prostate cancer DU145 cells,human bladder cancer T24 cells.CCK-BR expression was detected in the clinical cancer tissues including the stomach,breast,cervical,bladder,lung and liver and AGS and SGC-7901 cells by immuno-histochemical and immunocytochemical methods.Results CCK-BR mRNA and protein were express in the cytoplasm and cell mem-brane of the above 8 cell lines.Positive expression of CCK-BR was discovered in the above cancer tissues.Conclusion CCK-BR are expressed in a variety of human tumor tissues and cells.