Proliferation inhibition and apoptosis of human tongue cancer cell line Tca 8113 by andrographolide / 重庆医学

ABSTRACT

Objective To explore the effects of andrographolide(AD) on proliferation inhibition ,cells cycle distribution and the expression of Bax and Bcl‐2 mRNA in human tongue squamous cell carcinoma Tca8113 cells .Methods MTT was used to measure the levels of the proliferation of Tca8113 cells cultured with different concentraions of AD .Cell cycles were analyzed by flow cytom‐etry .RT‐polymerase chain reaction(RT‐PCR)technique was used to evaluate Bcl‐2 mRNA and Bax mRNA expression .Results An‐drographolide inhibited Tca8113 cells growth in a time and dose‐dependent manner ,the IC50 was 74 .66 μg/mL .A feature of apop‐tosis was observed under microscope .With a increase in concentration and increase in time ,the cells in G0/G1 phase increased from (39 .45 ± 0 .65)% to (63 .70 ± 0 .65)% ,the cells in S phase decreased from (56 .55 ± 0 .64)% to (32 .28 ± 0 .54)% .Statistics showed tha the cells arrested in G0/G1 phase .It was statistially significant in comparison with the control group (P< 0 .01) .AD could up‐regulate the experssion of Bax mRNA ,and AD could down‐regulate the experssion of Bcl‐2 mRNA .Conclusion An‐drographolide inhibited the growth of Tca8113 cells and increased the cell apoptosis .It may be promising as a new drug for treat‐ment of tongue squamous cell carcinoma .