Experimental study on transfection of humanβ-NGF recombinant plasmid into BMSCs of GFP transgenic mouse / 重庆医学
Chongqing Medicine
;
(36): 1161-1163,1167, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-599106
ABSTRACT
Objective To investigate the biological features of the mouse bone marrow stromal stem cells (BMSCs) transfected by humanβnerve growth factor(β-NGF) .Methods BMSCs of GFP transgenic mouse were isolated and cultured .Theβ-NGF re-combinant plasmid vectors were transferred into the cultured BMSCs by LipofectamineTM 2000 .The expression of β-NGF was detec-ted with ELISA .Results The β-NGF recombinant plasmid vectors were successfully transferred into BMSCs of GFP transgenic mouse ,the expression of β-NGF in transfected cells appeared .In addition ,the expression ofβ-NGF could effectively protect the BM-SCs which were injured in transfection process .Conclusion BMSCs of GFP transgenic mouse after transfecton with human β-NGF have the proliferation and differentiation capacity ,and possess the expression ability of β-NGF protein .
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chongqing Medicine
Year:
2014
Type:
Article
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