Experimental study of correlation between T-cell paradigm and bone loss induced by estrogen deficiency / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 870-873,878, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-599362
ABSTRACT
Objective:
To demonstrate the relationship between the Th 1/Th2/Th17/Treg paradigm and the bone loss induced by estrogen deficiency and looking for potential target for clinical treatment.Methods:
30 BALB/c mice were divided randomly into the normal control group , the sham operation group , and the ovariectomy group.The serum estradiol ( E2 ) was assessed by ELISA.Bone mineral density (BMD) of thigh bone was measured with dual energy X ray absorptiometry.Meanwhile,the T-cell subsets (Th1CD4+TNFα+, Th2 CD4+IL-4+, Th17 CD4+IL-17 A+, Treg CD4+CD25+Foxp3+) in spleen lymphocytes were analyzed by flow cytometry.Results:
Compared with the normal group and the sham operation group , both E2 and BMD in the ovariectomy group decreased significantly ( P<0.05 ).The percentage of Th 1 and Th17 subset increased while the percentage of Th 2 and Treg decreased significantly in ovariectomy mice compared with sham operation mice.Correlation analysis showed that BMD was positively related to E 2 level and the percentage of Th 2 and Treg subset;however ,BMD was negatively related to the percentage of Th 1 and Th17 subset ( P<0.05 ).Conclusion:
Conclusion:
T-cell paradigm was involved in the bone loss induced by estrogen deficiency.Modifying T-cell paradigm may become a potential target for reducing bone loss induced by estrogen deficiency .
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2014
Type:
Article
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