Expression of Tim-3 in hepatocellular carcinoma cells and its effect on cell proliferation and migration ability / 重庆医学

ABSTRACT

Objective To study the expression of T cell immunoglobulin mucin-3(Tim-3)in hepatocellular carcinoma(HCC)cell line and its influence on the oncobiological behavior of HCC cells.Methods The expression of Tim-3 mRNA and protein in human normal liver cell line L02 and HCC cell line HepG2 and SMMC7721 was assessed by FQ-PCR and Western blot.The siRNA Tim-3 fragments were designed to silence the Tim-3 gene expression in HepG2 cel1.HepG2 cells were transfected with siRNA by using LipofectamineTM 2000.The expression of Tim-3 protein was detected after transfection by Western blot to screen the effective siR-NA fragment.The proliferation and migration potential of hepG2 cells was evaluated after Tim-3 gene silence by the cell growth curve MTT assay and the wound healing assay.Results Both expressions of Tim-3 mRNA and protein in human HCC cell line HepG2 and SMMC7721 were significantly higher than those in normal liver cell line L02(P<0.05).After siRNA transfection,the protein expression of Tim-3 in experimental group was significantly lower than that of the control group.Compared with control group,the proliferative activity and the migration ability were decreased significantly.Conclusion Expressions of Tim-3 mRNA and protein are increased in HCC cell line.Tim-3 expression promotes HCC cell proliferation and migration.