Study on HLA-A,B,DRB1 high-resolution alleles polymorphism in Chongqing Han population / 重庆医学

ABSTRACT

Objective To analyze the HLA-A ,B and DRB1 alleles high-resolution polymorphism in Chongqing Han population . Methods The PCR-SSOP and PCR-SBT methods were applied for the HLA high-resolution genotyping of 2 067 unrelated healthy donors in the registry of Chongqing branch of Chinese National Marrow Donor Program (CMDP) .The allele frequencies of HLA-A , B and DRB1 were estimated by the direct counting method and the Hardy-Weinberg equilibrium inspection was performed by using the Arlequin software 3 .1 .Results 168 high-resolution alleles were detected out ,in which 42 alleles of A*1101 ,A*2402 ,A*0207 ,A*0201 and A*3303 at the HLA-A locus were observed with the frequencies greater than 0 .05 ;81 alleles were detected at HLA-B locus ,including B*4601 ,B*4001 ,B*5801 ,B*1301 and B*1502 with the frequencies greater than 0 .05 ;45 al-leles of DRB1*0901 ,DRB1*1501 ,DRB1*1202 ,DRB1*0803 and DRB1*1101 at the HLA-DR locus were observed with the frequencies greater than 0 .05 .Conclusion The data of the HLA-A ,B and DRB1allelic frequencies at high-resolution level in Chongqing Han population are obtained ,which provides the reliable reference data for the studies of anthropology ,forensic medi-cine ,transplantation matching and disease association .