Expression levels and effects of thioredoxin reductase-1 on non-small cell lung cancer / 临床与实验病理学杂志

ABSTRACT

Purpose To explore the expression of thioredoxin reductase-1 ( TrxR1 ) in non-small cell lung cancer and to observe the effects of TrxR1 on non-small cell lung cancer ( NSCLC) . Methods Immunohistochemistry was used to determine the expression of TrxR1 in 118 NSCLC tissues and 27 normal lung tissues, and to analyze its relationships with clinical pathological features. TrxR1 mR-NA levels were measured by quantitative RT-PCR in 13 cases of NSCLC and 4 cases of normal lung tissue. TrxR1 protein and its mR-NA levels were assessed by Western blotting and quantitative RT-PCR in normal lung epithelial cells and different lung cancer cell lines. After the silence TrxR1 expression in A549 cells, the changes of proliferation were compared. Results The data of immunohis-tochemistry and quantitative RT-PCR showed the overexpression of TrxR1 in NSCLC ( P <0. 05 ) . After using shRNA silencing of TrxR1 in lung cancer cells, cell proliferation changed significantly. Conclusion There are a high mRNA and protein levels of TrxR1 in NSCLC. TrxR1 may be a critical therapeutic target for the treatment in NSCLC.