The effects of p75NTR gene on the apoptosis of tongue squamous cell carcinoma Tca8113 cells / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 15-18,19, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-600642
ABSTRACT
Objective:
To study the effect of p75NTR gene on the apoptosis of tongue squamous cell carcinoma Tca8113 cells.Methods:
p75NTR +Tca8113 cells were isolated from Tca 8113 cell line and transfected by p75NTR siRNA using lipofectamine. p75NTR mRNA and protein expression was examined by real time RT-PCR and western blot respectively.Cell proliferation was stud-ied by MTT assay,cell apoptosis was examined by flow cytometry.Results:
Proliferation of p75NTR + cells was faster than that of p75NTR - cells.Transfection of p75NTR siRNA inhibited p75NTR mRNA and protein expression in p75NTR + Tca8113 cells,inhibi-ted the proliferation and increased the apoptosis of the cells.Conclusion:
p75NTR gene plays a role in the apoptosis of Tca8113 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS