Effect of Genistein combined with cyclosporine A on CXCR3 in rejection of cardiac allograft / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effect of Genistein combined with cyclosporine A on chemokine receptor CXCR3 in rejection of cardiac allograft in rats.Methods:Heterotopic cervical heart transplantation was performed from Wistar rats to SD rats by using cuff-technique.The SD rat recipients were randomly divided into 3 groups.No treatment was adopted in the acute rejection( AR) group.Rats in the cyclosporine A(CsA) group were treated with cyclosporine A after transplantion.Rats in CsA+Genistein(C+G) group were treated with Genistein combined with cyclosporine A after transplantion.All the cardiac allografts were harvested at 7th day, and made into tissue slices.The HE-staining was used to observe the pathology changes of the allograft myocardia.And the expression of CXCR3 was detected with immunehistochemistry and Western blot.Results:The expression of CXCR3 strongly positively expressed in AR group.The degree of myocardial inflammation in C+G group and the expression of CXCR3 were much lower than the other two groups.Conclusion:Genistein combined with cyclosporine A can significantly relieve rejection of cardiac allograft by inhibiting the ex-pression of CXCR3.