Th17 Responses Are Not Induced in Dextran Sodium Sulfate Model of Acute Colitis
Immune Network
;
: 416-419, 2011.
Article
in English
| WPRIM
| ID: wpr-60130
ABSTRACT
Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-gamma (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sodium
/
Sulfates
/
T-Lymphocytes
/
Inflammatory Bowel Diseases
/
Dextrans
/
Interleukin-4
/
Colitis
/
Colon
/
Th1 Cells
/
Interleukin-17
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Immune Network
Year:
2011
Type:
Article
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