Disruption of URA-3 gene of a paclitaxel-producing fungus N8 / 国际生物医学工程杂志
International Journal of Biomedical Engineering
;
(6): 257-261,封3, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-601617
ABSTRACT
Objective In order to get genetic markers,an auxotrophic paclitaxel-producing fungus named Pestalotiopsis malicola N8 strain was isolated by genetic modification.Methods Based on the homologous recombination,URA-3 which is the key gene for uracil synthetic route of Pestalotiopsis malicola N8 strain was knocked out.The transformants were screened by minimal medium with the combination of 5-fluoroorotic acid (5-FOA) and uracil.Results The results showed that the uracil auxotrophic strain was able to grow in the minimal medium containing 5-FOA and uracil while the wild type strain was not.Conclusions The uracil auxotrophic strain can be used as a new selection marker for future gene function studies of N8 strain.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Journal of Biomedical Engineering
Year:
2014
Type:
Article
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