Construction of a Multi-Copy Secretory Expression Vector and Hirudin Expression in Pichia Pastoris / 天津医药
Tianjin Medical Journal
;
(12): 112-114,前插2, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-601692
ABSTRACT
Objective:
To construct suitable vectors for the secretory expression of hirudin in Pichia pastoris.Methods:
The α-facor-hirudin gene was amplified from pPIC9-hirudin by PCR and sub-cloned into PA0815.The multi-copy recombinant plasmid pA0815-(α-Hirudin)n was constructed.The recombinant was transformed into P.pastoris strain GS115 for induction expression and then the activity of secreted products was identified.Results:
A new multi-copy vector pA0815-(αHirudin)n was successfully constructed and was capable of secreting recombinant hirudin efficiently,which was confirmed respectively by PCR and SDS-PAGE.The products possessed the activity of thrombin inhibitor.Conclusion:
This result offers efficient P.pastoris stains for mass production of biological active hirudin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tianjin Medical Journal
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS