The Growth Inhibition Induced by SUMO-1 siRNA in Hepatocellular Carcinoma Cell Line SMMC-7721 / 天津医药
Tianjin Medical Journal
;
(12): 4-6,后插1, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-601725
ABSTRACT
Objective:
To study the efficiency of silencing small ubiquitin-like modifier-1(SUMO-1) induced by siRNA in hepatocellular carcinoma cell line SMMC-7721 and the growth inhibition of SMMC-7721 thereof.Methods:
The SUMO-1 siRNA was transfected into SMMC-7721 by means of lipofectamine~(TM) 2000. The silencing efficiency of SUMO-1 was examined by RT-PCR and western blot. The cell growth and cell cycle were examined by MTT and flow cytometry(FCM). The cell apoptosis was detected by DeadEnd~(TM) Colorimetric TUNEL System.Results:
The siRNA could significantly silence the expression of SUMO-1 in SMMC-7721.The maximal silencing rate was utmost 73.43% at 48 hours after being transfected SUMO-1 siRNA. MTT assay revealed that the cell line grew more slowly. FCM result showed that the number of G_2 stage cells was increased significantly. But apoptosis cells were not found by TUNNEL assay.Conclusion:
SiRNA is a good manner to silence the expression of SUMO-1 in SMMC-7721 in vitro. Owing to the growth inhibition induced by SUMO-1 siRNA, SUMO-1 plays an important role in development of SMMC-7721.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tianjin Medical Journal
Year:
2010
Type:
Article
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