Study of Reversing Invasion of Human Gastric Cancer Cell Line BGC-823 by Targeting Angiopoietin-1 Using siRNA / 天津医药
Tianjin Medical Journal
;
(12): 752-754,849, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-601876
ABSTRACT
Objective To knock down angiopoietin-1 expression in human gastric cancer cell line BGC-823 and to observe its effect of reversing tumor invasion. Methods siRNA sequence fragments was designed to target angiopoietin-1 and transferred into human gastric cancer cell line BGC-823. RT-PCR was used to assess the transcription level of angio-poietin-1 mRNA, then western blot and immunofluorescence were used to examine the expression level of three invasion-as-sociated proteins include integrinβ1, CD44V6 and Ang-1. Cell adhesion ability was evaluated by cell adhesion assay and cell invation was determined by matrigel and transwell plastic dual-chamber culture system. Results Ang-1 mRNA was knocked down by siRNA showed by RT-PCR. The expression of integrinβ1, CD44V6 and Ang-1 were significantly lower than control group(P<0.05), so did the cellular adhesion and invasion abilities(P<0.05). Conclusion Knocking down angiopoietin-1 by siRNA can reverse invasion of human gastric cancer cell line BGC-823 and may provide new ideas and reference for gene therapy of gastric cancer in the future.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tianjin Medical Journal
Year:
2014
Type:
Article
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