A Study on Dermatophagoides pteronyssinus Induced Cytokines Gene Expression in Peripheral Blood Mononuclear Cells / 소아알레르기및호흡기학회지
Pediatric Allergy and Respiratory Disease
; : 109-121, 2001.
Article
in Ko
| WPRIM
| ID: wpr-60190
Responsible library:
WPRO
ABSTRACT
PURPOSE: Cytokines are known to play a major role in mediating many of the immunological and pathological findings of allergic disease. Many studies on allergen-specific T cell clones isolated from atopic individuals have shown that these cells produce cytokines of the Th2 phenotype. The purpose of the present study was to investigate cytokine patterns of T lymphocyte induced by Dermatophagoides pteronyssinus in atopic asthma. METHODS: Freshly isolated peripheral blood mononuclear cells of Dp(+) asthmatic children, Dp(-) asthmatic children, nonasthmatic children, and normal adults were stimulated with Dp antigen and examined for the induction of IFN-gamma, IL-2, IL-4, and IL-5 mRNA using RT-PCR. RESULTS: The lymphoproliferative response to crude Dp antigen was maximized at Dp concentration of 2.5 microgram/mL. The lymphoproliferative response was low in children including Dp(+) asthmatics, Dp(-) asthmatics and nonasthmatics, but that of normal adults was high. The expression of IFN-gamma was not so different in each group, but the expression of IL-4 was least in adults, and that of IL-5 was most prominent in Dp(+) asthmatic children. In adult healthy group, IFN-gamma expression without IL-4 and IFN-gamma without IL-5 was significantly higher than those in children. IL-5 expression with or without IFN-gamma was the highest in Dp(+) asthmatic children. IL-4 and IL-5 or IL-5 without IL-4 expression were observed more frequently in Dp(+) asthmatic children than in any other groups. Th2 response was most prominent in Dp(+) asthmatics, and Th1 response was most prominent in normal adult group. CONCLUSION: In conclusion, we observed significantly prominent Th2 response in Dp(+) asthmatic children, especially that of IL-5.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
/
Asthma
/
RNA, Messenger
/
Lymphocytes
/
Gene Expression
/
Cytokines
/
Interleukin-4
/
Interleukin-5
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Interleukin-2
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Negotiating
Limits:
Adult
/
Child
/
Humans
Language:
Ko
Journal:
Pediatric Allergy and Respiratory Disease
Year:
2001
Type:
Article