Effects of edaravone on expression of caspase-3 and caspase-12 in juvenile rat hippocampus after status convulsion / 中国病理生理杂志

ABSTRACT

[ ABSTRACT] AIM:To investigate the effect of edaravone ( ED) on the expression of caspase-3 and caspase-12 in the juvenile rat hippocampus after status convulsion ( SC) .METHODS: Juvenile male Sprague-Dawley rats were ran-domly divided into normal saline ( NS) control group, SC group and ED treatment group.The rats in each group were fur-ther divided into 5 subgroups according to different time points.The rats in SC group were kindled into epilepsy by lithium-pilocarpine chemical method.The protein expression of caspase-3 and caspase-12 was determined by immunohistochemistry methods.The mRNA expression of caspase-3 and caspase-12 was detected by RT-PCR.RESULTS:(1) The IA value of caspase-3 positive cells in 24~72 h SC group increased compared with NS group.With ED intervention, the IA value of caspase-3 positive cells decreased as compared with 48~72 h SC group.The results of RT-PCR showed that the mRNA ex-pression of caspase-3 was similar to the changes of protein.( 2 ) The results of immunohistochemistry showed that the IA value of caspase-12 positive cells in 12~72 h SC group increased compared with NS group.With ED intervention, the IA value of caspase-12 positive cells decreased as compared with 24~72 h SC group.The results of RT-PCR showed that the mRNA expression of caspase-12 was similar to the changes of protein.( 3 ) In ED group, Ⅴ grade convulsion was lower than that in SC group, and the latent period of seizures in ED group was significantly longer than that in SC group.CON-CLUSIONEdaravone inhibits the expression of caspase-3 and caspase-12 in pilocarpine-induced seizures in rat hippo-campus, suggesting that edaravone has protective effect against the damage caused by status convulsion.