Effects of Celecoxib on the Cell Migration, MMP-9 and VEGF Expression in Human Gastric Cancer SGC-7901 / 天津医药

ABSTRACT

Objective To observe selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on the cell migration, MMP-9 and VEGF expression of human gastric cancer SGC-7901. Methods In vitro culture of SGC-7901 cells, the cells were divided into blank control group and celecoxib treatment group (different concentrations of celecoxib 25, 50 and 100μmol/L). SGC-7901 cells were treated with celecoxib for 24 h. The effect of celecoxib on cell migration of SGC-7901 was detected by the damage repair experiment. RT-PCR assay was used to detect the effect of celecoxib on MMP-9 mRNA and VEGF mRNA expressions in SGC-7901 cells. Results The migration distance of SGC-7901 cells was decreased with the increased concentration of celecoxib. There were significant differences between medium-dose and high-dose celecoxib groups and the control group. RT-PCR assay showed that MMP-9 mRNA and VEGF mRNA expressions in gastric cancer SGC-7901 were significantly decreased with the increased concentration of celecoxib, and there was a significant difference compared with those of control group (P＜0.05). Conclusion Celecoxib can inhibit migration of human gastric cancer SGC-7901 cells, which may be related to the inhibition of COX-2 downstream of MMP-9 and VEGF expressions.