Simultaneous Determination of Gallic Acid and Berberine Hydrochloride in Dihuang Sprays by HPLC / 中国药师
China Pharmacist
;
(12): 1682-1683,1686, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-602079
ABSTRACT
Objective:
To establish an HPLC method for the determination of gallic acid and berberine hydrochloride in Dihuang sprays.Methods:
A ZORBAX SB-C18 column (250 mm × 4. 6 mm,5 μm) was used, the mobile phase was acetonitrile-0. 1% phos-phoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the column temperature was 30℃, and the detection wavelength wassetat270nm.Results:
Thelinearrangeofgallicacidandberberinehydrochloridewas20.36-305.40 μg·ml-1(r=0.9996) and 20.08-301.20 μg·ml-1(r=0.999 8), respectively. The average recovery was 98.9%(RSD =1.3%) and 98.5%(RSD =1. 5%) , respectively.Conclusion:
The method is simple, reliable and accurate, which can be used in the determination of gallic acid and berberine hydrochloride of Dihuang sprays.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2014
Type:
Article
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