Experimental studies on inhibitory effects of total flavonoids in Scorzonera austriaca wild on hepatitis B virus in vitro / 中国生化药物杂志

ABSTRACT

Objective To investigate anti-HBV effect of total flavonoids in Scorzonera austriaca wild (TFSA) in vitro.Methods MTT assay was used to observe the effect of TFSA on HepG2.2.15 cells, ELISA assay was used to detect the inhibition on HBsAg and HBeAg secretion from HepG2.2.15 cells and RTFQ-PCR assay was used to detect the inhibition rates of HBV-DNA.ResuIts The TC50 of TFSA on HepG2.2.15 cells was 0.603 mg/mL . TFSA significantly reduced the content of HBsAg and HBeAg and the numbers of HBV-DNA in the HepG2.2.15 cell cultural supernatants under nontoxic concentrations (0.062, 0.125, 0.250 mg/mL), and the maximal inhibitory rate was 89%, 33% and 43%, respectively. ConcIusion TFSA have anti-HBV effects in vitro.