Hemocyanin is an important high-molecular-weight allergen of Eriocheir sinensis / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1375-1379,1388, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-602405
ABSTRACT
Objective:
To prove hemocyanin is an important high-molecular-weight allergen from Eriocheir sinensis.Methods:
The proteins were extracted from Eriocheir sinensis tissue with lysate extraction method .The protein components were analyzed by SDS-PAGE,two-dimensional electrophoresis and Western blot.Serum IgE from allergic donors identified a candidate protein,which was char-acterized by MALDI-TOF/TOF.The immune property of the candidate protein was tested using Dot-blot.Results:
By SDS-PAGE and two-dimensional electrophoresis analysis,we prove that the native protein components were completely.According to the Western blot result,at least 18 components could react with the positive serum.Among them, two 70-80 kD proteins had the same isoelectric point.So,may be they were the same protein.MALDI-TOF/TOF analysis results showed that the suspected proteins were hemocyanin.A sensitization frequency of 61%was observed in Eriocheir sinensis patients by Dot-blot.Conclusion:
Hemocyanin was identified as an important high-molecular-weight allergen from Eriocheir sinensis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2015
Type:
Article
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