Influence of Mycoplasma pneumoniae infection on the expression of interleukin-18 in BALB/c mice / 国际检验医学杂志

ABSTRACT

Objective To investigate the expression of interluekin(IL)-18 in BALB/c mice serum and bronchoalveolar fuid infec-ted by mycoplasma pneumonia(MP) and to clarify the relationship between IL-18 and MP infection .Methods A total of 72 BABL/c mice were randomly divided into control group ,MP infection group and treatment group ,each group with 24 mice .MP infection models were established with ripping cultured MP into nasal cavity of mice .Clarithromycin was used at the fifth day in the treat-ment group .The blood of eyeball and bronchoalveolar lavage fluid were collected at the first ,fifth ,ninth day in the three groups ,and detected IL-18 .At the third ,seventh ,forth ,twenty-first ,30th day ,one mice was selected to isolate lung tissue ,and make pathologi-cal section to observe lesion degree .Results Pathological section indicated that lung tissue of mice in the control group had no in-flammatory response ,but lung tissue in the M P infection group had serious inflammatory response ,lung tissue in the treatment group at seventh day had mild inflammatory response .Compared with that of the control group ,the IL-18 in the serum and bron-choalveolar lavage fluid significantly increased at the first ,fifth ,ninth day ,the differences were statistically significant (tfirst day =10 .08 ,tfifth day =9 .14 ,tninth day = 10 .59 ,P< 0 .05) .The IL-18 levels in the serum and bronchoalveolar lavage fluid at fifth day and ninth day in the treatment group were significantly lower than those of the MP infection group(tfifth day =12 .96 ,tninth day =17 .95 ,P<0 .05) .Conclusion MP infection causes IL-18 level increasing ,it is effective to treat with clarithromycin ,and IL-18 might correlate with pneumonia severity .