Plasmid DNA and Random Amplified Polymorphic DNA (RAPD) Analyses of Vancomycin-Resistant Enterococcus faecium Isolates / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 379-385, 1998.
Article
in Korean
| WPRIM
| ID: wpr-60265
ABSTRACT
BACKGROUND:
Vancomycin-resistant enterococci (VRE) have been increasingly reported worldwide. The understanding of VRE dissemination in the hospital requires a molecular typing of the strains. VRE appeared recently in Chonnam University Hospital. The purpose of this study is to analyse the strains for their genetic relatedness.METHODS:
Nine vancomycin-resistant E. faecium isolates, collected from six patients during 1995-1996 in Chonnam University Hospital, were typed using plasmid DNA and RAPD analyses. The plasmid DNA of the isolates was obtained by a alkaline lysis method. For RAPD, eight random primers were used. The cluster analysis was performed by NTSYS-pc (numerical taxonomy system and multivariate analysis system, version 1.50, Applied Biostatistics Inc., CA).RESULTS:
Nine VRE isolates were separated into two different molecular types (group A and B) by the plasmid DNA patterns, which were agreed with the RAPDresults:
the isolates of each group showed the same plasmid DNA patterns and high similarity values in the RAPD analysis. Group A was consisted of two strains isolated from two patients who were admitted at the same room in May 1995. Seven strains of group B were isolated from four patients in the different wards during June 1995 to June 1996.CONCLUSIONS:
Nine VRE isolates from six patients were typed to two groups by plasmid DNA or RAPD analysis. These results suggested the intrahospital spread of two clonal strains of vancomycin-resistant E. faecium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
DNA
/
Biostatistics
/
Multivariate Analysis
/
Classification
/
Enterococcus faecium
/
Enterococcus
/
Molecular Typing
Type of study:
Controlled clinical trial
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
1998
Type:
Article
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