Determination of Levofloxacin Hydrochloride Ophthalmic in situ Gel by HPLC / 中国药师
China Pharmacist
;
(12): 1987-1988,1989, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-602736
ABSTRACT
Objective:
To establish an HPLC method for the determination of levofloxacin in levofloxacin hydrochloride ophthalmic in situ gel.Methods:
The chromatographic column was Agilent Zorbax C18(150 mm ×4.6 mm,5μm). The mobile phase consisted of hexane sulfonic acid sodium solution-methanol(72∶28). The flow rate was 1. 0 ml·min-1. The detection wavelength was 293 nm. The chromatographic column temperature was 40℃. The injection volume was 10 μl.Results:
The calibration curve was linear within the range of 0. 040 3-0. 403 0 mg·ml-1(r=1. 000 0) for levofloxacin. The average recovery was 99. 8% (RSD=1. 08%,n=9).Conclusion:
The method is accurate, simple and rapid, and suitable for the content determination of levofloxacin in levofloxacin hydro-chloride ophthalmic in situ gel.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2015
Type:
Article
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