Clinical application and evaluation of Sysmex XE-5000 automated blood cell analyser for detecting nucleated red blood cells / 国际检验医学杂志

ABSTRACT

Objective To evaluate the performance of XE‐5000 automated blood cell analyser for detecting nucleated red blood cell (NRBC) in peripheral blood and investigate its clinical application value .Methods The intra‐assay imprecision ,carryover rate , and linear range of the analyser were evaluated .The absolute NRBC count and percentage of NRBC of 137 blood specimens (NRBC‐positive according to the DIFF channel of the analyser) were determined in the NRBC channel of the analyser ,and the percentage of NRBC of these blood specimens were determined by using microscope method as well .Differences between the two methods were analysed by using SPSS18 .0 statistic software .Results The intra‐assay imprecision of the analyser for detecting absolute NRBC count in specimens with high ,moderate ,and low Q‐flag values were 2 .10% ,3 .26% and 11 .62% ,respectively ,and the imprecision for detecting percentage of NRBC were 3 .79% ,5 .80% and 13 .33% ,respectively .The carryover rates of the analyser for detecting absolute NRBC count and percentage of NRBC were 0 .51% and 0 .26% ,respectively .At the range of (0~18)× 109/L ,the absolute NRBC count detected by using the analyser showed good linearityY=1 .048 6X+0 .189 6(r=0 .999 1) .There were no statistically significant differences between the analyser and microscope for detecting percentage of NRBC(P=0 .716) ,and showed good corre‐lationY=1 .150 2X+0 .626 1(r=0 .967 0) .Conclusion The XE‐5000 automated blood cell analyser could completely replace the traditional microscope for clinically classifying and counting NRBCs .