Effects of glycyrrhizin on the expressions of IL-2 and IL-4 in HaCaT cells / 天津医药

ABSTRACT

Objective To observe the effect of glycyrrhizin (CG) on the proliferation and the expressions of interleukin (IL)-2, IL-4 in human keratinocytes cell line HaCaT cells, and to investigate the therapeutic mechanism of CG in the pa?tients with psoriasis. Methods The HaCaT cells were divided into four groupsblank group (without CG), low concentration group (50μL CG/mL cell supernatant), medium concentration group (100μL CG/mL cell supernatant) and high concentra?tion group (200μL CG/mL cell supernatant). Methyl thiazolyl tetrazolium (MTT) assay was performed to evaluate the prolifer?ation of HaCaT cells that were treated with CG at different concentrations respectively for 24 hours. The contents of IL-2 and IL-4 in HaCaT cells were examined by enzyme linked immunosorbent assay (ELISA). Results Compared with the blank group, there were obviously inhibitory effects in the proliferation of HaCaT cells treated by low,medium and high concentra?tions of CG for 24 hours. There was no significant difference in IL-2 level between low concentration CG group and blank group (ng/L234.51±17.98 vs 225.31±16.23, P>0.05). After 24 hours of intervention with CG of medium and high concen?trations, the expression levels of IL-2 was significantly lower in HaCaT cells than that in blank group and low concentration group. The IL-2 level was significantly lower in high concentration group than that in medium concentration group (ng/L188.99±19.22 vs 208.49±18.40, P<0.05). At the same condition, the secretion of IL-4 in HaCaT cells was significantly up-regulated in high concentration group than that in other three groups (ng/L45.67±10.29 vs 37.62±3.90, 39.68±6.08, 43.85± 10.26, P<0.05). Conclusion Glycyrrhizin may suppress the proliferation of human skin keratinocytes by regulating secre?tion of cytokines IL-2 and IL-4.